What are the Challenges in Using Biologic Agents in Histology?
Despite their utility, the use of biologic agents in histology presents several challenges:
- Antigen Retrieval: Formalin fixation can mask antigens, requiring the use of antigen retrieval techniques to unmask these sites. - Cross-Reactivity: Antibodies may bind to unintended targets, leading to false-positive results. - Reproducibility: Ensuring consistent results across different experiments and laboratories can be difficult.
