Despite its importance, tissue segmentation poses several challenges:
Variability in Staining: Different staining techniques and variations in staining intensity can affect segmentation accuracy. Complex Tissue Structures: The intricate and heterogeneous nature of tissues makes it difficult to delineate boundaries accurately. Noise and Artifacts: Histological images often contain noise and artifacts, which can interfere with segmentation algorithms. Computational Cost: Advanced automated techniques require substantial computational resources, which may not be readily available in all settings.
