The preparation of tissue samples is a critical step in empirical histology methods. The process generally includes:
Fixation: Preserves tissue by using chemicals that prevent decay and maintain cellular integrity. Embedding: Involves encasing the tissue in a solid medium, such as paraffin wax, to facilitate slicing. Sectioning: Thin slices of the embedded tissue are cut using a microtome, enabling examination under a microscope. Staining: The tissue sections are stained to enhance contrast and visualize different components clearly.
