Capturing high-quality histological images presents several challenges: - Artifact Formation: Artifacts can arise during tissue processing, leading to distortions that may obscure true tissue architecture. - Resolution Limits: Each imaging technique has its resolution limits, which can affect the level of detail captured. - Sample Integrity: Maintaining the integrity of delicate tissue samples throughout preparation and imaging is critical but challenging. - Interpretation: Accurate interpretation of histological images requires significant expertise and experience.