Tissue preparation is a critical step in histology captures, involving several stages: 1. Fixation: Preserves tissue morphology and prevents degradation by using chemical fixatives like formalin. 2. Embedding: Tissues are embedded in paraffin or resin to provide support for thin sectioning. 3. Sectioning: Thin slices of tissue, typically 4-5 micrometers thick, are cut using a microtome. 4. Staining: Sections are stained with various dyes, such as Hematoxylin and Eosin (H&E), to enhance contrast and highlight different tissue components. 5. Mounting: Stained sections are mounted on glass slides for examination under a microscope.
