Despite its importance, cell tracking comes with several challenges:
Photobleaching: Prolonged exposure to light can cause fluorescent labels to fade, reducing the signal over time. Phototoxicity: High-intensity light used for imaging can damage cells, affecting their behavior. Multiplexing: Tracking multiple cell types simultaneously requires distinct labels that do not interfere with each other. Data Complexity: The sheer volume of data generated from time-lapse imaging can be overwhelming and requires robust computational tools for analysis.
