What are the Challenges Associated with Multiplex PCR?
Despite its advantages, multiplex PCR also presents several challenges:
Primer Design: Designing compatible primers that do not interfere with each other is crucial. This requires careful optimization to ensure efficient amplification of all targets. Optimization: Reaction conditions need to be meticulously optimized to balance the amplification efficiencies of different targets. Cross-Reactivity: There is a risk of primer-dimer formation and non-specific amplification, which can complicate the interpretation of results.
