What are the Challenges Associated with Multiplex Immunohistochemistry?
Despite its advantages, mIHC also presents certain challenges:
Antibody Cross-Reactivity: The use of multiple antibodies can lead to cross-reactivity, which may result in non-specific binding and false-positive results. Signal Overlap: When using multiple fluorophores, there is a risk of signal overlap, which can complicate the interpretation of results. Optimization: The protocol for mIHC needs to be carefully optimized for each set of markers and tissue type, which can be time-consuming and complex. Data Analysis: The large amount of data generated by mIHC requires sophisticated software and analytical tools to accurately interpret the results.
