What Are the Best Practices to Ensure Adequate Fixation?
To avoid inadequate fixation, consider the following best practices:
Optimal Fixative Choice: Select a fixative appropriate for the tissue type and the intended downstream applications. Proper Fixation Time: Follow recommended fixation times, typically ranging from a few hours to overnight, depending on the tissue and fixative. Fixative Volume: Ensure that the volume of fixative is at least 10 times the volume of the tissue to allow for proper penetration. Temperature Control: Maintain an appropriate temperature to facilitate adequate fixation.
