There are several advantages to using sticky ends:
1. Increased Efficiency: Sticky ends increase the efficiency of DNA ligation because the complementary overhangs can easily find each other and form stable hydrogen bonds.
2. Specificity: The specific nature of the overhangs ensures that only complementary sequences will anneal, reducing the likelihood of non-specific ligation.
3. Versatility: Sticky ends can be used in various applications, from basic molecular cloning to advanced techniques in genetic engineering and tissue modification.
