There are several advantages to using Alexa Fluor 647 in histological applications:
High Sensitivity: Its high quantum yield ensures that even low-abundance targets can be detected. Low Photobleaching: The dye is resistant to photobleaching, allowing for prolonged observation periods. Compatibility: It is compatible with a range of other fluorophores, enabling multiplexing in complex assays. Reduced Background: The far-red emission reduces interference from tissue autofluorescence.