One of the primary advantages of CISH is that it allows for the visualization of target sequences within the context of tissue morphology, which is preserved during the procedure. This localization is particularly important for understanding the spatial distribution of genetic alterations within heterogeneous tissues. Unlike FISH, CISH does not require a fluorescence microscope, as the signals are visible under a standard bright-field microscope. This makes CISH more accessible and easier to interpret for many laboratories.
