Several strategies can be employed to minimize the artifacts caused by superposition:
Proper Sectioning: Using microtomes to obtain thin, consistent tissue sections can significantly reduce the chances of superposition. Optimal Mounting: Ensuring that tissue samples are properly mounted on slides without folds or overlaps can help achieve clearer results. Advanced Imaging Techniques: Techniques such as confocal microscopy or 3D reconstruction can provide more accurate representations of tissue architecture by reducing the impact of superposition.
