Splicing enhancers are short sequences of nucleotides within the pre-mRNA that play a critical role in the accurate and efficient removal of introns during the process of RNA splicing. These sequences are vital for the proper formation of mature mRNA, which is then translated into functional proteins. Splicing enhancers can be classified into two main types: exonic splicing enhancers (ESEs) and intronic splicing enhancers (ISEs).