To address some of the limitations of GFP, several variants have been developed:
- Enhanced GFP (EGFP): Modified for brighter fluorescence and greater stability. - Yellow Fluorescent Protein (YFP): Shifted emission spectrum to yellow, useful for multi-color imaging. - Cyan Fluorescent Protein (CFP): Emission spectrum shifted to cyan, also useful for multi-color imaging. - Red Fluorescent Protein (RFP): Provides a red fluorescence, minimizing spectral overlap with GFP.
