In addition to traditional light microscopy, histologists use several advanced techniques to study tissues in greater detail:
Fluorescence Microscopy: This technique uses fluorescent dyes or proteins to label specific cellular components. Immunohistochemistry (IHC): IHC employs antibodies to detect specific proteins, providing information about protein expression and localization. Electron Microscopy: This method offers high-resolution images of tissue ultrastructure, including transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Confocal Microscopy: Confocal microscopy provides high-resolution and three-dimensional images of tissues.