The required section thickness can vary depending on the type of tissue and the specific application:
Paraffin-embedded tissues: Typically, sections are cut at 4-6 µm. Frozen sections: Usually, sections are cut at 5-10 µm due to the softer nature of the tissue. Special Stains: Certain stains may require different thicknesses, for instance, immunohistochemistry often requires thinner sections (3-4 µm) for better antigen-antibody interactions.
