Several methods are used for amplification in histology, including:
Polymerase Chain Reaction (PCR): This technique is often used to amplify DNA sequences, allowing for the detection of specific genetic material within a sample. Immunohistochemistry (IHC): This method uses antibodies to bind specifically to target antigens. Secondary antibodies, which are conjugated to enzymes or fluorophores, amplify the signal. In Situ Hybridization (ISH): This technique involves using labeled complementary DNA or RNA probes to detect specific nucleic acid sequences, with subsequent signal amplification through enzymatic reactions or fluorescent tags.
