Common chemiluminescent substrates include luminol, acridinium esters, and dioxetanes. Luminol is widely used in Western blotting and Immunohistochemistry due to its high sensitivity and ease of use. Acridinium esters are often used in ELISA assays because they provide stable and intense luminescent signals. Dioxetanes are used for their ability to produce prolonged light emission, which is beneficial for imaging applications.
