In histology, chemiluminescence is primarily used in techniques like Western blotting, ELISA (Enzyme-Linked Immunosorbent Assay), and Immunohistochemistry. These techniques involve the binding of antibodies to target molecules within tissue sections. Upon binding, an enzyme such as horseradish peroxidase (HRP) catalyzes a reaction that produces a luminescent signal, which can be detected and quantified.
