Several challenges can arise during the verification process:
Sample Quality: Poor-quality samples due to improper collection or handling can compromise the results. Staining Artifacts: Inconsistent or inadequate staining can lead to misinterpretation of histological features. Observer Variability: Differences in interpretation between pathologists or researchers can affect the consistency of the results. Technical Errors: Errors during embedding, sectioning, or microscopy can introduce artifacts or distortions.