Antifade reagents are solutions containing compounds that protect fluorescent molecules from photobleaching. Common components include DABCO (1,4-diazabicyclo[2.2.2]octane), P-phenylenediamine (PPD), and various proprietary blends offered by commercial suppliers. These reagents work by scavenging reactive oxygen species (ROS) generated during illumination, thereby preventing the chemical degradation of fluorescent dyes.
