What are Alternative Methods to Thymidine Labeling?
While thymidine labeling is a powerful tool, there are alternative methods to study cell proliferation, including:
Ki-67 staining: A protein expressed in all active phases of the cell cycle except G0, used as a cellular proliferation marker. Flow cytometry: Uses fluorescent dyes to measure DNA content and cell cycle distribution in a population of cells. EdU labeling: A thymidine analogue similar to BrdU but detected using a click chemistry reaction, offering a safer and faster alternative.
