Fixation: The tissue sample is fixed using a fixative to preserve its structure. Dehydration: The sample is dehydrated through a series of alcohol solutions to remove water content. Clearing: The sample is cleared using a clearing agent to make it transparent and compatible with the embedding medium. Infiltration: The tissue is infiltrated with the embedding medium, usually paraffin wax. Embedding: The infiltrated tissue is placed in the embedding mold and additional paraffin is poured to form a solid block. Cooling: The mold is cooled rapidly to solidify the paraffin, encapsulating the tissue in a firm block.
