Preventing alignment problems involves meticulous attention to detail during each step of the histology workflow:
1. Embedding Techniques: Ensure tissues are correctly oriented during embedding. Use orientation markers if necessary. 2. Microtome Calibration: Regularly calibrate and maintain the microtome to ensure precise sectioning. 3. Consistent Section Thickness: Maintain uniform section thickness to avoid discrepancies in alignment. 4. Proper Mounting: Carefully mount sections onto slides, ensuring they are flat and well-aligned.
