How to Prepare Tissue Samples for Brightfield Microscopy?
Proper preparation of tissue samples is essential for effective brightfield microscopy. The process generally involves:
1. Fixation: Preserving the tissue to prevent degradation, often using formaldehyde or glutaraldehyde. 2. Embedding: Infiltrating tissues with paraffin or resin to provide support during sectioning. 3. Sectioning: Cutting thin slices of the embedded tissue using a microtome. 4. Staining: Applying appropriate stains to highlight different tissue components.
