Preparation and use of basic dyes involve several steps to ensure optimal staining:
Dye Preparation: Dissolve the dye in an appropriate solvent, usually water or ethanol, to the desired concentration. Tissue Preparation: Fix the tissue sample to preserve its structure and prevent degradation. Staining Procedure: Apply the dye to the tissue sample for a specified duration, followed by washing to remove excess dye. Microscopic Examination: Examine the stained tissue under a microscope to observe the stained cellular components.
