How to Optimize the Use of Enzyme-Linked Antibodies?
1. Blocking Non-Specific Binding: Use blocking agents like bovine serum albumin (BSA) or normal serum to reduce non-specific binding. 2. Choosing the Right Controls: Include both positive and negative controls to validate the specificity and sensitivity of the assay. 3. Substrate Selection: Choose appropriate substrates based on the enzyme used and the detection method. Some substrates provide better contrast and are more stable than others.
