To minimize tissue artifacts, several steps can be taken:
1. Proper Fixation: Use appropriate fixatives and ensure tissues are promptly fixed after collection. 2. Careful Handling: Handle tissues gently and use sharp microtome blades to avoid mechanical damage. 3. Temperature Control: Maintain optimal temperatures during embedding and sectioning to avoid thermal artifacts. 4. Solution Preparation: Ensure correct pH and concentration of chemical solutions to prevent chemical artifacts.
