Fixation is the first and one of the most critical steps in tissue preparation. The choice of fixative and the duration of fixation can significantly impact the quality of the histological preparation. Here are some tips:
Select the appropriate fixative based on the tissue type and the specific histological analysis required. Ensure the tissue sample is fully submerged in the fixative to prevent any air bubbles, which can cause artifacts. Follow the recommended fixation time and temperature to avoid over-fixation or under-fixation.
