Improving detection sensitivity involves optimizing various steps in the histological workflow: - Optimizing Fixation: Using appropriate fixation methods and times to preserve tissue morphology and antigenicity. - Selecting High-Quality Reagents: Employing high-specificity antibodies and high-quality stains. - Enhancing Microscopy Techniques: Utilizing advanced imaging techniques and ensuring proper calibration and maintenance of microscopes. - Implementing Amplification Techniques: Using signal amplification methods to enhance weak signals. - Standardizing Protocols: Developing and following standardized protocols to reduce variability and improve reproducibility.
