Identification of staining artifacts involves careful examination of tissue sections under the microscope. Common clues include irregular staining patterns, unusual colors, and structural distortions that do not correlate with known tissue anatomy. Preventing artifacts requires meticulous attention to detail throughout the entire histological process:
Ensure prompt and appropriate fixation of tissues. Use high-quality reagents and follow standardized protocols. Monitor environmental conditions, such as temperature and humidity, during processing and staining. Regularly maintain and calibrate histological equipment. Train personnel in proper techniques and troubleshooting.
