How to Choose the Right Fluorophores and Chromogens?
Choosing the appropriate fluorophores or chromogens is critical for successful multi-color labeling. Consider the following:
- Spectral Properties: Ensure that the emission spectra of the fluorophores do not overlap excessively to avoid signal bleed-through. - Photostability: Select fluorophores that are resistant to photobleaching for long-term imaging. - Compatibility: Opt for chromogens that can be used together without causing non-specific staining or cross-reactivity.
