The application process generally involves the following steps: 1. Dehydration: Ensure that the tissue section is completely dehydrated through a series of alcohol washes followed by a clearing agent like xylene. 2. Mounting: Place a small drop of the non-aqueous mounting medium on the tissue section. 3. Coverslipping: Carefully lower a coverslip onto the drop to avoid air bubbles. 4. Drying: Allow the slide to dry completely, which can vary from a few hours to overnight depending on the medium used.
