VEGF expression in colorectal cancer is quantified using several techniques. Immunohistochemistry (IHC) is the most common method, but other techniques such as Western blotting and ELISA (Enzyme-Linked Immunosorbent Assay) can also be used. IHC provides a visual representation of VEGF localization, while Western blotting and ELISA quantify the protein levels in tissue extracts.