Histological diagnosis of ulceration involves several steps:
1. Biopsy: A tissue sample is taken from the ulcerated area. 2. Fixation: The tissue is fixed in a solution like formalin to preserve cellular details. 3. Sectioning: Thin sections of the tissue are cut using a microtome. 4. Staining: Common stains like Hematoxylin and Eosin (H&E) are used to highlight cellular structures. 5. Microscopic Examination: Pathologists examine the stained sections under a microscope to assess the extent and nature of the ulceration.
