Research on type I collagen involves various methods to understand its structure, function, and role in diseases: - Biochemical Assays: Techniques such as Western blotting and ELISA to quantify collagen levels. - Genetic Studies: Investigating mutations in COL1A1 and COL1A2 genes and their effects on collagen synthesis. - Cell Culture Models: Using fibroblasts and other cells to study collagen production and its regulation. - Animal Models: Creating transgenic or knockout mice to understand the functional implications of collagen mutations.
