After fixation, tissues undergo a series of steps to prepare them for microscopic examination. These steps include: 1. Dehydration: Removal of water from the tissue using graded alcohols. 2. Clearing: Replacing alcohol with a substance like xylene that is miscible with paraffin. 3. Infiltration: Impregnating the tissue with molten paraffin wax. 4. Embedding: Encasing the tissue in a block of paraffin wax to provide support for sectioning.
