How is Tissue Prepared for Histological Examination?
The process of preparing tissue for histological examination involves several steps:
Fixation: Preserves the tissue and prevents autolysis. Formaldehyde is commonly used as a fixative. Embedding: The tissue is embedded in a solid medium, usually paraffin wax, to allow thin sections to be cut. Sectioning: Thin slices of the tissue are cut using a microtome. Staining: Stains such as Hematoxylin and Eosin (H&E) are applied to highlight different tissue components.
