3D imaging in histology is performed by acquiring a series of 2D images at different depths within the tissue. These images are then digitally reconstructed to form a 3D model. The process involves several key steps:
Sample Preparation: Tissue samples are prepared using standard histological techniques, including fixation, embedding, and sectioning. Image Acquisition: Sequential 2D images are captured using imaging modalities like confocal or multiphoton microscopy. Digital Reconstruction: Specialized software is used to align and reconstruct the 2D images into a 3D model. Analysis: The reconstructed 3D model is analyzed to study the spatial relationships and structures within the tissue.
