The process of thin sectioning involves several steps:
Fixation: The tissue sample is preserved using chemical fixatives to prevent decay and maintain structural integrity. Embedding: The fixed tissue is embedded in a solid medium, usually paraffin wax or resin, to provide support during slicing. Sectioning: Using a microtome, the embedded tissue is sliced into thin sections. The thickness can be adjusted based on the requirement of the study. Mounting: The thin sections are placed on glass slides to be stained and examined under a microscope. Staining: Various dyes and stains are applied to highlight different cellular components, making them visible under a microscope.
