The study of the RRP involves a combination of techniques in [molecular biology](https://), [electrophysiology](https://), and [microscopy](https://). Electrophysiological methods, such as patch-clamp recordings, are used to measure synaptic responses and the kinetics of neurotransmitter release. Advanced imaging techniques, such as [fluorescence microscopy](https://) and [electron microscopy](https://), are employed to visualize vesicle dynamics and the structure of presynaptic terminals.
