Histological techniques used to study the lipid barrier include:
1. Electron Microscopy: Provides detailed images of the lipid bilayer and associated structures at a molecular level. 2. Lipid Staining: Techniques like Oil Red O, Sudan Black, and Nile Red staining are used to visualize lipids in tissue sections. 3. Immunohistochemistry: Employs antibodies to detect specific lipid-associated proteins within tissue samples. 4. Fluorescence Microscopy: Utilizes fluorescent dyes that bind to lipids, allowing for the visualization of lipid distribution in cells and tissues.
