In histology, the G2 checkpoint can be studied by analyzing tissue samples for markers of DNA damage and cell cycle progression. Techniques such as [immunohistochemistry](), [Western blotting](), and [fluorescence microscopy]() can be employed to detect the presence and activation state of proteins like p53, Cyclin B, and phosphorylated histone H2AX (γ-H2AX), which is an indicator of DNA damage. Additionally, the use of [flow cytometry]() allows for the assessment of cell cycle distribution and the identification of cells in the G2 phase.
