The preparation of a frozen section involves several steps:
1. Tissue Sampling: A small piece of tissue is removed from the patient during surgery. 2. Freezing: The tissue sample is quickly frozen using a cryostat, a machine that maintains extremely low temperatures. 3. Sectioning: The frozen tissue is then sliced into thin sections using a microtome within the cryostat. 4. Staining: The sections are stained, typically with Hematoxylin and Eosin (H&E), to distinguish different cellular components. 5. Microscopy: The stained sections are examined under a microscope by a pathologist.
