Tetrazolium salts are commonly used in histological studies to assess cell viability, proliferation, and cytotoxicity. The reduction of tetrazolium salts to formazan is an indicator of metabolic activity and is often employed in assays such as the MTT assay, XTT assay, and WST assay. These assays measure the ability of cellular enzymes to reduce tetrazolium salts, thus providing a quantitative measure of viable cells.