The staining process with Sudan Black involves several steps:
Fixation: Tissue samples are fixed using a fixative, such as formalin, to preserve their structure. Sectioning: The fixed tissues are embedded in a medium like paraffin and sectioned into thin slices using a microtome. Staining: The tissue sections are stained with Sudan Black solution, allowing the dye to bind to the lipids present. Rinsing: Excess dye is washed away, typically using water or an alcohol solution. Mounting: The stained sections are mounted on slides and covered with a coverslip for microscopic examination.
