Several techniques are employed to study spatial context:
Histological Staining: Techniques like Hematoxylin and Eosin staining help distinguish different tissues and cells based on their morphology and color. Immunohistochemistry: This technique uses antibodies to detect specific proteins, allowing for the visualization of specific cell types and their spatial distribution. In Situ Hybridization: This method detects specific nucleic acid sequences in tissue sections, providing spatial information about gene expression. Three-Dimensional Imaging: Techniques such as confocal microscopy and MRI create 3D images of tissues, offering a comprehensive view of their spatial arrangement.