single cell proteomics

How is Single Cell Proteomics Performed?

Single cell proteomics involves several advanced techniques. Initially, single cells are isolated using methods such as fluorescence-activated cell sorting (FACS) or microfluidics. Following isolation, proteins from individual cells are extracted, often necessitating miniaturized and highly sensitive assays. Techniques like mass spectrometry (MS), immunohistochemistry (IHC), and cytometry by time-of-flight (CyTOF) are then employed to identify and quantify the proteins.

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