Histological identification of segmental scarring involves several techniques:
Light microscopy: This is the primary method for observing tissue architecture and identifying areas of scarring. Special stains such as Masson's trichrome or Periodic acid-Schiff (PAS) can be used to highlight fibrotic tissue. Immunohistochemistry: This technique can be used to detect specific proteins associated with fibrosis, such as collagen or fibronectin. Electron microscopy: Though less commonly used, electron microscopy can provide detailed images of the ultrastructure of scarred tissue, offering insights into cellular and extracellular matrix changes.
